Thermo Scientific Random Hexamers Increase Sensitivity and Yield in Reverse Transcription
Date Posted: Tuesday, August 11, 2009
Thermo Fisher Scientific offers life scientists Thermo Scientific Random Hexamers as a tool for the enhanced reverse transcription and amplification of messenger RNA (mRNA).
The company says that, random hexamers are ideal when working with short fragments, such as in QRT-PCR, by increasing the number of binding sites on the RNA, allowing for an increase in reaction sensitivity and yield of cDNA.
Thermo Scientific Random Hexamers are used as primers for mRNA with or without a poly-A tail. These truly random oligo deoxyribonucleotides increase sensitivity and yield of reverse transcription reactions as well as generating a complete pool of cDNA. Random hexamers consist of six bases in a randomly generated sequence, to allow for annealing on all species of RNA.
Thermo Scientific Random Hexamers are available at a concentration of 400 ng/ml and in aliquots of 40ml and 100ml. They are also available in Thermo Scientific Verso RT and QRT-PCR kits to offer a complete reverse transcription solution.
Further Information: http://www.thermofisher.com
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