A Nanofluidic Real Time PCR Array for the 7900HT PCR System
Jing Wang, Senior Chip Scientist, Fluidigm Corporation
Date Posted: Monday, October 19, 2009
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Launch presentation
About the speaker
On March 2003, Jing Wang received his Ph.D. in BioMEMS from Louisiana Tech University. Currently, Dr. Jing Wang is a Senior Chip Scientist at Fluidigm Corporation at South San Francisco, CA, where he has contributed several successes since March, 2006. Dr. Wang’s major invents at Fluidigm includes carry-slug loading, on-chip check valve, 32x32 Array and other smart chips. Prior to that, Dr. Wang was a researcher at the Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania. His researching was focused on integrated bio-chip with cell lysis, DNA purification, on-chip PCR and lateral flow detection.
Abstract
Fluidigm Corporation has introduced a number of integrated nanofluidic platforms for high throughput, low cost, flexible system operation with reduced reaction volume and required labor. These are developed to run on the Fluidigm BioMark System. The nanofluidic array discussed here, offers the same benefits but runs on the AB 7900HT real-time system. The array provides 24-sample by 16-assay inputs in an SBS 384 well format. It enables real-time Polymerase Chain Reaction (q PCR) with far fewer pipetting steps compared to a regular 384 well plate. The array automates the delivery of assay specific components and samples to each of the 384 500 nL reaction volumes, thereby enabling good mix ratio precision, all with less than half the total volume of a TaqMan Low Density Array (TLDA card). The array also offers assay flexibility as the user can decide which assay chemistries to load at the time of run. The array performs gene expression assays with Ct precision, PCR efficiency, and gene expression level consistency within and between chips comparable to that of microtiter plates. The array also delivers 384 well plate quality genotyping results that a micro tilter card could not achieve. Further, the array can be directly loaded into the 7900HT PCR system without any instrument or software modification. This nanofluidic array could be a complete replacement for 384 well plate or TLDA card.
Launch presentation