GoTaq® qPCR Master Mix

GoTaq® qPCR Master Mix is a new qPCR reagent that contains a new DNA binding dye that exhibits greater fluorescence than SYBR® Green. Combined with the GoTaq® Hot Start and an optimized buffer, GoTaq® qPCR Master Mix provides robust real-time PCR with increased reliability, reproducibility and sensitivity.

GoTaq® qPCR Master Mix offers:

• Earlier Cq and detection of low copy targets
• Enhanced stability for automated setup
• Direct substitute for SYBR® Green I products
• The robust, reliable performance of GoTaq® Hot Start
• The Promega PCR Performance Guarantee

GoTaq is a registered trademark of Promega Corporation. SYBR is a registered trademark of Molecular Probes, Inc.

Detection of GAPDH from indicated amounts of cDNA using GoTaq® qPCR Master Mix and Company A’s SYBR® Green qPCR Master Mix.

qPCR with single copy detection: The GAPDH gene was amplified and detected from a 10-fold serial dilutions of human gDNA.

Further Information: http://www.promega.com/applications/pcr/qpcr.htm

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